Tsakona C P, Goldstone A H
Haematology Department, University College Hospital, London.
Clin Lab Haematol. 1992;14(4):273-80. doi: 10.1111/j.1365-2257.1992.tb00102.x.
The pattern of changes in neutrophil myeloperoxidase (MPO) before, during and after bacteraemia was studied in 34 patients recovering from autologous bone marrow transplantation for relapsed Hodgkin's disease and non Hodgkin's lymphomas. Thirteen patients received haemopoietic growth factors (7 received M-CSF, 3 received G-CSF and 3 GM-CSF). The mean peroxidase index (MPXI) produced as part of a routine FBC performed by a flow cytochemistry blood autoanalyser (Technicon H*1) was used as a parameter to assess the MPO and subsequently the azurophil degranulation. The manufacturer's normal values for MPXI range from -10 to +10. Median MPXI on the day of documented bacteraemia was just below normal in the control and M-CSF groups (-10.8 and -8.9 respectively), but it was much below normal in the G-CSF (-16.5, P < 0.05) and even lower in the GM-CSF group (-39.6, P < 0.02); this correlated well with the decreased bacteraemia incidence in the last two groups. Although contact of neutrophils with bacterial chemoattractants resulted in primary degranulation in all groups, the pattern of changes in MPO content was different, suggesting that neutrophils primed in vivo with various haemopoietins respond to the challenge of microbial agents via different pathways.
对34例因复发性霍奇金病和非霍奇金淋巴瘤接受自体骨髓移植后正在康复的患者，研究了菌血症发生前、期间及之后中性粒细胞髓过氧化物酶（MPO）的变化模式。13例患者接受了造血生长因子（7例接受M-CSF，3例接受G-CSF，3例接受GM-CSF）。通过流式细胞化学血液自动分析仪（Technicon H*1）进行的常规全血细胞计数（FBC）得出的平均过氧化物酶指数（MPXI），被用作评估MPO以及随后嗜天青颗粒脱粒的一个参数。该仪器制造商给出的MPXI正常范围是-10至+10。在记录有菌血症当天，对照组和M-CSF组的MPXI中位数略低于正常水平（分别为-10.8和-8.9），但G-CSF组远低于正常水平（-16.5，P<0.05），GM-CSF组更低（-39.6，P<0.02）；这与后两组中菌血症发生率降低密切相关。尽管中性粒细胞与细菌趋化因子接触会导致所有组出现初级脱粒，但MPO含量的变化模式不同，这表明在体内用各种造血素预处理的中性粒细胞通过不同途径应对微生物制剂的挑战。
