Gulevsky A K, Grischenko V I, Zagnoiko V I, Shchenyavsky I I, Ilyasova E N
Institute for Problems of Cryobiology and Cryomedicine, Ukrainian Academy of Sciences, Kharkov, Ukraine.
Cryobiology. 1992 Dec;29(6):679-84. doi: 10.1016/0011-2240(92)90071-9.
Synthesis of proteins and RNA has been studied by means of labeled precursors of 14C-labeled amino acids and [14C]orotic acid in liver cells of hibernating, arousing, and active ground squirrels (Citellus undulatus). The investigations indicate that cycloheximide (CHI)-inhibited protein synthesis in liver cells of hibernating ground squirrels may occur on preexisting polyribosomes. A low level of protein synthesis in this state results not only from the Arrhenius dependence, but also from the dissociation of polyribosomes into monosomes. During arousal there is an intensive assembly of polyribosomes. This occurs even with the inhibition of RNA synthesis by actinomycin D, testifying to the presence of a pool of iRNA in liver cells, which is necessary to activate translation. The intensity of protein synthesis in arousal is also influenced by the activation of transcription.
利用14C标记的氨基酸和[14C]乳清酸的标记前体，对冬眠、觉醒和活跃状态的地松鼠（Citellus undulatus）肝细胞中的蛋白质和RNA合成进行了研究。研究表明，环己酰亚胺（CHI）抑制的冬眠地松鼠肝细胞中的蛋白质合成可能发生在预先存在的多核糖体上。这种状态下蛋白质合成水平较低不仅是由于阿累尼乌斯依赖性，还由于多核糖体解离为单核糖体。在觉醒过程中，多核糖体有大量组装。即使在放线菌素D抑制RNA合成的情况下也会发生这种情况，这证明肝细胞中存在iRNA池，这是激活翻译所必需的。觉醒过程中蛋白质合成的强度也受到转录激活的影响。
