Cardoso João C, Power Deborah M, Elgar Greg, Clark Melody S
Fugu Genomics Group, MRC-HGMP Resource Centre, Genome Campus, Hinxton, Cambridge, UK.
DNA Seq. 2003 Apr;14(2):129-33. doi: 10.1080/1042517031000081142.
The genomic organisation of a putative GHRH receptor gene has been characterised in the teleost fish, Fugu rubripes. It comprises 13 exons and is at least 1.2 x larger than previously described vertebrate GHRH receptors. Sequence conservation with other vertebrate GHRH receptors is highest over the seven transmembrane domains with preservation of 8 conserved cysteines and an N-glycosylation site in the N-terminal region, which are important for receptor activation. Database mining revealed a further putative GHRH receptor in Fugu, with phylogenetic tree topology indicating that this was potentially a teleost-specific duplication event. This is the first time that the genomic organisation of a GHRH receptor gene has been characterised from a nonmammalian vertebrate.
在硬骨鱼红鳍东方鲀中，已对一个假定的生长激素释放激素（GHRH）受体基因的基因组结构进行了表征。它由13个外显子组成，比先前描述的脊椎动物GHRH受体至少大1.2倍。与其他脊椎动物GHRH受体的序列保守性在七个跨膜结构域中最高，在N端区域保留了8个保守的半胱氨酸和一个N-糖基化位点，这些对于受体激活很重要。数据库挖掘揭示了红鳍东方鲀中另一个假定的GHRH受体，系统发育树拓扑结构表明这可能是硬骨鱼特有的复制事件。这是首次从非哺乳类脊椎动物中表征GHRH受体基因的基因组结构。
