Lü Y, Tang D, Yu L, Ding Y, Liu L, Guo Y
Department of Urology, First Hospital, Beijing Medical University, China.
Zhonghua Wai Ke Za Zhi. 2000 Apr;38(4):294-6.
To investigate the expression of microsatellite instability in transitional cell carcinoma of bladder and to detect its diagnostic value.
Urine samples from 35 patients with transitional cell carcinoma of bladder (TCC) were analyzed by PCR method. 25 patients were followed up to detect microsatellite instability in their urine sediments.
Microsatellite changes (including MSI and LOH) were detected in 88.6% of urine sediments (31 of 35 patients). Microsatellite changes were detected in urine sediments of 10 of 12 patients with tumor recurrence, in which the existence of tumor cells in the urine of 3 patients had been correctly predicted before cystoscopic evidence from 3 to 6 months. CONCLUSION Microsatellite analysis of urine sediment may be a novel and potentially clinical tool for the diagnosis and follow-up of bladder cancer patients.
探讨微卫星不稳定性在膀胱移行细胞癌中的表达情况，并检测其诊断价值。
采用聚合酶链反应（PCR）法分析35例膀胱移行细胞癌（TCC）患者的尿液样本。对其中25例患者进行随访，检测其尿沉渣中的微卫星不稳定性。
88.6%（35例中的31例）的尿沉渣检测到微卫星改变（包括微卫星高度不稳定和杂合性缺失）。12例肿瘤复发患者中有10例尿沉渣检测到微卫星改变，其中3例患者在膀胱镜检查证据出现前3至6个月，尿液中肿瘤细胞的存在就已被正确预测。结论：尿沉渣微卫星分析可能是一种用于膀胱癌患者诊断和随访的新型且具有潜在临床应用价值的工具。
