Munoz G E, Marshall S H
Laboratorio de Genética Molecular, Universidad Católica de Valparaíso, Chile.
Cell Mol Biol (Noisy-le-grand). 1992 Aug-Sep;38(5-6):629-33.
Human platelets, either purified or plasma-enriched, are activated when exposed to free L-phosphotyrosine. Physical aggregation is similar to that induced by collagen, although with distinctive biochemical features. Among these, a mobility shift of a GTP-binding protein specifically recognized by an anti-Ki-v-ras monoclonal antibody and an altered pattern of low molecular weight phosphorylated polypeptides are the most outstanding features. Since free phosphotyrosine is detected in platelets extracts, its role as a new signal transducer as well as its putative modulating action over protein phosphorylation are discussed.
纯化的或富含血浆的人血小板在暴露于游离L-磷酸酪氨酸时会被激活。物理聚集与胶原蛋白诱导的聚集相似，尽管具有独特的生化特征。其中，抗Ki-v-ras单克隆抗体特异性识别的GTP结合蛋白的迁移率变化以及低分子量磷酸化多肽模式的改变是最突出的特征。由于在血小板提取物中检测到游离磷酸酪氨酸，因此讨论了其作为新信号转导分子的作用及其对蛋白质磷酸化的假定调节作用。
