Bunch T A, Brower D L
Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721.
Development. 1992 Sep;116(1):239-47. doi: 10.1242/dev.116.1.239.
Integrins are a family of transmembrane glycoproteins that mediate cell-matrix and cell-cell interactions. We have transfected cultured Drosophila cells with genes that express the Drosophila PS2 integrin. We demonstrate that this integrin is expressed on the surface of the cells and can mediate cell spreading on an undefined component of fetal calf serum or on the purified vertebrate matrix molecules vitronectin and fibronectin. Additionally, PS2 integrin can cause cell spreading on RGD peptide. The spreading on matrix components or RGD peptide can be inhibited by soluble RGD peptide and is dependent on divalent cations.
整合素是一族跨膜糖蛋白，可介导细胞与基质以及细胞与细胞之间的相互作用。我们已用表达果蝇PS2整合素的基因转染了培养的果蝇细胞。我们证明，这种整合素在细胞表面表达，并且能够介导细胞在胎牛血清的一种未明确成分上，或在纯化的脊椎动物基质分子玻连蛋白和纤连蛋白上的铺展。此外，PS2整合素可使细胞在RGD肽上发生铺展。在基质成分或RGD肽上的铺展可被可溶性RGD肽抑制，并且依赖于二价阳离子。
