Kawano Shin-ichi, Murakita Hiroyuki, Yamamoto Eiichi, Asakawa Naoki
Analytical Applications Department, Shimadzu Corporation, 380-1 Horiyamashita, Hadano, Kanagawa 259-1304, Japan.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jul 15;792(1):49-54. doi: 10.1016/s1570-0232(03)00309-x.
A novel methylcellulose-immobilized reversed-phase pretreatment column (MC-ODS) for column switching liquid chromatography-mass spectrometry (LC-MS) was investigated to improve recovery and durability. Pretreatment and analytical conditions were optimized so that high throughput and high selectivity was ensured during mass spectrometric analysis. Analytical runs, including deproteinization and gradient LC analysis, were conducted in a 6-min cycle. As a consequence, recoveries for test drugs (metoprolol, propranolol, lidocaine, dibucaine, bupivacaine) were greater than 90% and more than 300 plasma samples spiked with target compounds were directly injected and measured without compromising MS detection or system performance.
为提高回收率和耐用性，研究了一种用于柱切换液相色谱-质谱联用（LC-MS）的新型甲基纤维素固定反相预处理柱（MC-ODS）。对预处理和分析条件进行了优化，以确保在质谱分析过程中具有高通量和高选择性。包括去蛋白和梯度液相色谱分析在内的分析运行以6分钟的周期进行。结果，测试药物（美托洛尔、普萘洛尔、利多卡因、丁卡因、布比卡因）的回收率大于90%，并且直接进样并测定了300多个添加了目标化合物的血浆样品，而不会影响质谱检测或系统性能。
