Sagach V F, Zhukova A V, Braquet P
A. A. Bogomoletz Institute of Physiology, Academy of Science of Ukraine, Kiev.
J Cardiovasc Pharmacol. 1992;20 Suppl 12:S85-9. doi: 10.1097/00005344-199204002-00025.
In experiments on anesthetized dogs, the effect of intracoronary administration of different doses of platelet-activating factor (PAF) has been investigated. One of the branches of the left coronary artery was catheterized via the main carotid artery. The coronary artery was autoperfused with blood from the subclavian artery. Intracoronary administration of small doses of PAF (100 and 200 ng/kg) caused a dose-dependent decrease in coronary resistance (CR) and an increase in coronary blood flow (CF). When 200 ng/kg of PAF was used, these changes were 30 and 34% vs. control, respectively. Dose increase to 300 ng/kg led to a biphasic reaction. Intracoronary administration of larger PAF doses produced opposite effects, i.e., increase in CR and decrease in CF. Chemical de-endothelialization by saponin (5 mg/5 ml, 1.5-2 min) transformed coronary dilation to small doses of PAF to constriction. The CR rose from 2.8 +/- 0.3 to 5.6 +/- 0.9 mm Hg/ml/min (p < 0.01) and CF dropped two-fold. The PAF receptor antagonists BN 52021 (6 mg/kg) and WEB 2086 (3 mg/kg) completely abolished PAF-induced coronary reactions. Hence, PAF-induced vasodilation of the canine coronary circulation is endothelium dependent.
在对麻醉犬进行的实验中，研究了冠状动脉内给予不同剂量血小板活化因子（PAF）的效果。通过颈总动脉将左冠状动脉的一个分支插入导管。冠状动脉用来自锁骨下动脉的血液进行自身灌注。冠状动脉内给予小剂量的PAF（100和200 ng/kg）导致冠状动脉阻力（CR）呈剂量依赖性降低，冠状动脉血流量（CF）增加。当使用200 ng/kg的PAF时，与对照组相比，这些变化分别为30%和34%。剂量增加到300 ng/kg导致双相反应。冠状动脉内给予更大剂量的PAF产生相反的效果，即CR增加和CF降低。用皂苷进行化学去内皮（5 mg/5 ml，1.5 - 2分钟）将小剂量PAF引起的冠状动脉扩张转变为收缩。CR从2.8 +/- 0.3升高到5.6 +/- 0.9 mmHg/ml/min（p < 0.01），CF下降了两倍。PAF受体拮抗剂BN 52021（6 mg/kg）和WEB 2086（3 mg/kg）完全消除了PAF诱导的冠状动脉反应。因此，PAF诱导的犬冠状动脉循环血管舒张是内皮依赖性的。
