Ohta Kazutoku, Ohashi Masayoshi, Jin Ji-Ye, Takeuchi Toyohide, Fujimoto Chuzo, Choi Seong-Ho, Ryoo Jae-Jeong, Lee Kwang-Pill
Ceramics Research Institute, National Institute of Advanced Industrial Science and Technology, 2266-98 Anagahora, Sinoshidami, Moriyama-ku, Nagoya 463-8560, Japan.
J Chromatogr A. 2003 May 16;997(1-2):117-25. doi: 10.1016/s0021-9673(03)00110-9.
The application of various hydrophilic cation-exchange resins for high-performance liquid chromatography (sulfonated silica gel: TSKgel SP-2SW, carboxylated silica gel: TSKgel CM-2SW, sulfonated polymethacrylate resin: TSKgel SP-5PW, carboxylated polymethacrylate resins: TSKgel CM-5PW and TSKgel OA-Pak A) as stationary phases in ion-exclusion chromatography for C1-C7 aliphatic carboxylic acids (formic, acetic, propionic, butyric, isovaleric, valeric, isocaproic, caproic, 2-methylhexanoic and heptanoic acids) and benzenecarboxylic acids (pyromellitic, trimellitic, hemimellitic, o-phthalic, m-phthalic, p-phthalic, benzoic, salicylic acids and phenol) was carried out using diluted sulfuric acid as the eluent. Silica-based cation-exchange resins (TSKgel SP-2SW and TSKgel CM-2SW) were very suitable for the ion-exclusion chromatographic separation of these benzenecarboxylic acids. Excellent simultaneous separation of these benzenecarboxylic acids was achieved on a TSKgel SP-2SW column (150 x 6 mm I.D.) in 17 min using a 2.5 mM sulfuric acid at pH 2.4 as the eluent. Polymethacrylate-based cation-exchange resins (TSKgel SP-5PW, TSKgel CM-5PW and TSKgel OA-Pak A) acted as advanced stationary phases for the ion-exclusion chromatographic separation of these C1-C7 aliphatic carboxylic acids. Excellent simultaneous separation of these C1-C7 acids was achieved on a TSKgel CM-5PW column (150 x 6 mm I.D.) in 32 min using a 0.05 mM sulfuric acid at pH 4.0 as the eluent.
将各种亲水性阳离子交换树脂（磺化硅胶：TSKgel SP - 2SW、羧化硅胶：TSKgel CM - 2SW、磺化聚甲基丙烯酸酯树脂：TSKgel SP - 5PW、羧化聚甲基丙烯酸酯树脂：TSKgel CM - 5PW和TSKgel OA - Pak A）用作高效液相色谱的固定相，用于离子排斥色谱法分离C1 - C7脂肪族羧酸（甲酸、乙酸、丙酸、丁酸、异戊酸、戊酸、异己酸、己酸、2 - 甲基己酸和庚酸）和苯羧酸（均苯四甲酸、偏苯三酸、偏苯三甲酸、邻苯二甲酸、间苯二甲酸、对苯二甲酸、苯甲酸、水杨酸和苯酚），采用稀硫酸作为洗脱剂。硅基阳离子交换树脂（TSKgel SP - 2SW和TSKgel CM - 2SW）非常适合这些苯羧酸的离子排斥色谱分离。使用pH为2.4的2.5 mM硫酸作为洗脱剂，在TSKgel SP - 2SW柱（内径150×6 mm）上17分钟内实现了这些苯羧酸的出色同时分离。聚甲基丙烯酸酯基阳离子交换树脂（TSKgel SP - 5PW、TSKgel CM - 5PW和TSKgel OA - Pak A）作为分离这些C1 - C7脂肪族羧酸的先进固定相。使用pH为4.0的0.05 mM硫酸作为洗脱剂，在TSKgel CM - 5PW柱（内径150×6 mm）上32分钟内实现了这些C1 - C7酸的出色同时分离。
