Mitov I, Georgiev G, Ivanova R, Petrov D, Levterova V, Strahilov D
Institute of Infectious and Parasitic Diseases, Sofia, Bulgaria.
Zentralbl Bakteriol. 1992 Oct;277(3):345-56. doi: 10.1016/s0934-8840(11)80913-8.
Four murine hybridoma clones secreting monoclonal antibodies (mAb) directed against lipopolysaccharide (LPS) antigen of S. newington, serogroup E1, were obtained after a fusion of spleen cells of mice immunized with formaldehyde-killed bacteria and mouse myeloma cells of the X63-Ag8.653 line. Antigen binding properties and specificity of the mAb were studied in bacterial agglutination tests, passive hemolysis and its inhibition, passive hemagglutination tests, passive hemolysis and its inhibition, passive hemagglutination and immunoenzyme tests (ELISA and immunoblotting). Three of the mAb (24E6, 29E1 and 45F6) were agglutinating and were active in all tests used, while mAb 31H12 did not agglutinate bacteria but revealed a high reactivity in the immunoenzyme reactions. It was found that the mAb reacted with LPS and Salmonella strains from serogroup E (E1, E2, E3 and E4) as well as from serogroups C (C1 and C4), F and S thus showing that the O3 antigen possesses more than one epitope, one of which is represented on the LPS antigens of the serovars from the cross-reacting groups mentioned. According to the known chemical the most probable recognized epitope consists of mannose with beta-linkage to the next monosaccharide residue in the LPS chain.
用甲醛灭活的纽因顿沙门氏菌（血清群E1）免疫小鼠的脾细胞与X63-Ag8.653系小鼠骨髓瘤细胞融合后，获得了4个分泌针对纽因顿沙门氏菌脂多糖（LPS）抗原的单克隆抗体（mAb）的小鼠杂交瘤克隆。通过细菌凝集试验、被动溶血及其抑制试验、被动血凝试验、被动溶血及其抑制试验、被动血凝试验和免疫酶试验（ELISA和免疫印迹）研究了mAb的抗原结合特性和特异性。其中3个mAb（24E6、29E1和45F6）具有凝集作用，在所有使用的试验中均有活性，而mAb 31H12不凝集细菌，但在免疫酶反应中显示出高反应性。发现这些mAb与血清群E（E1、E2、E3和E4）以及血清群C（C1和C4）、F和S的LPS和沙门氏菌菌株发生反应，因此表明O3抗原具有多个表位，其中一个表位存在于上述交叉反应群血清型的LPS抗原上。根据已知的化学结构，最可能识别的表位由与LPS链中下一个单糖残基以β键相连的甘露糖组成。
