模拟体外循环中的一氧化氮与炎症反应

Nitric oxide and inflammatory response in simulated extracorporeal circulation.

作者信息

Lahtinen M, Melki V, Adamik B, Khamis H, Borowiec J W

机构信息

Department of Cardiothoracic Surgery, Uppsala University Hospital, Sweden.

出版信息

Thorac Cardiovasc Surg. 2003 Jun;51(3):130-7. doi: 10.1055/s-2003-40318.

DOI:10.1055/s-2003-40318

PMID:12833201

Abstract

BACKGROUND

Simulated extracorporeal circulation (SECC) induces inflammatory reaction. Nitric oxide (NO) has pro-and anti-inflammatory properties. NO role in SECC-related inflammatory response is unclear. The aim of this study was to clarify if NO affects the foreign-surface induced leukocyte activation during SECC.

METHODS

Human blood was circulated through SECC during 3 hours. Control group C was ventilated with oxygen/air mixture and the study group with oxygen/air mixture and NO. Leukocyte activation was measured as serum levels of myeloperoxidase (MPO), human neutrophil lipocalin (HNL), lactoferrin (LF), interleukin-1-beta (IL-1 beta) and interleukin-10 (IL-10). Oxygen free radical production capacity was evaluated with chemiluminescence. NO metabolites nitrite/nitrate were estimated in serum.

RESULTS

Leukocyte granule release increased over time. Addition of NO significantly increased MPO, HNL and LF release. The average difference increased with SECC duration. NO addition did not significantly affect measured interleukins concentration or oxygen free radical production capacity. NO metabolites increased significantly in the NO circuits.

CONCLUSIONS

Results indicate that NO addition during SECC is pro-inflammatory and has no effect on oxygen free radical production and interleukin release.

摘要

背景

模拟体外循环（SECC）可引发炎症反应。一氧化氮（NO）具有促炎和抗炎特性。NO在SECC相关炎症反应中的作用尚不清楚。本研究的目的是阐明NO是否影响SECC期间异物表面诱导的白细胞活化。

方法

将人体血液在SECC中循环3小时。对照组C用氧气/空气混合物通气，研究组用氧气/空气混合物和NO通气。通过测定血清中髓过氧化物酶（MPO）、人中性粒细胞脂质运载蛋白（HNL）、乳铁蛋白（LF）、白细胞介素-1-β（IL-1β）和白细胞介素-10（IL-10）的水平来检测白细胞活化情况。用化学发光法评估氧自由基产生能力。测定血清中NO代谢产物亚硝酸盐/硝酸盐含量。