Boschman G A, Rens W, Manders E M, Slater R M, Versteeg R, Aten J A
Laboratory of Radiobiology, University of Amsterdam, The Netherlands.
Genes Chromosomes Cancer. 1992 Nov;5(4):375-84. doi: 10.1002/gcc.2870050413.
Bivariate flow karyotyping can be used for the detection of recurrent chromosome abnormalities in tumor cells. For this purpose 2 cell lines originally derived from Ewing's sarcomas and 4 cell lines from peripheral neuroectodermal tumors were used. The characteristic t(11;22) was known to be present in 5 cell lines. The remaining cell line was known to have a variant t(2;11;22;21) translocation. Metaphase chromosomes were stained with the fluorescent dyes Hoechst 33258 and Chromomycin A3 and analyzed subsequently using bivariate flow cytometry. The resulting bivariate flow karyotypes of the tumor cells were normalized by a standardized procedure using a computerized method and compared with a reference flow karyotype of normal chromosomes. In 5 cell lines two recurring abnormal chromosome peaks were identified at positions expected for the der(11) and der(22) chromosomes characteristic for the reciprocal t(11;22)(q24;q12). In the remaining cell line with the variant t(2;11;22;21), only the peak representing the der(22) was identifiable. It is concluded that bivariate flow karyotyping can be used for the semiautomated detection of recurrent translocations and the assessment of their variability among different tumors.
双变量流式核型分析可用于检测肿瘤细胞中反复出现的染色体异常。为此，使用了2个最初源自尤因肉瘤的细胞系和4个源自外周神经外胚层肿瘤的细胞系。已知5个细胞系中存在特征性的t(11;22)。已知其余细胞系具有变异的t(2;11;22;21)易位。中期染色体用荧光染料Hoechst 33258和嗜铬霉素A3染色，随后使用双变量流式细胞术进行分析。肿瘤细胞的双变量流式核型通过标准化程序使用计算机方法进行归一化，并与正常染色体的参考流式核型进行比较。在5个细胞系中，在预期的der(11)和der(22)染色体位置上鉴定出两个反复出现的异常染色体峰，这是相互t(11;22)(q24;q12)的特征。在具有变异t(2;11;22;21)的其余细胞系中，仅可识别代表der(22)的峰。结论是双变量流式核型分析可用于反复易位的半自动检测及其在不同肿瘤之间变异性的评估。
