Flügel C, Kinne R W, Streilein J W, Lütjen-Drecoll E
Department of Anatomy, University of Erlangen-Nürnberg, Germany.
Curr Eye Res. 1992 Dec;11(12):1173-83. doi: 10.3109/02713689208999542.
Cells of bone marrow origin that normally occupy the stroma of the murine iris and ciliary body have been implicated in the immune phenomenon, anterior chamber associated immune deviation (ACAID). Following injection of antigen into the anterior chamber, cells of this type deliver an ACAID inducing signal into the systemic circulation, presumably through the outflow tract. In an effort to identify such cells in man, anterior chambers of 34 human donor eyes of different age groups were stained immunocytochemically with monoclonal antibodies directed at HLA class II molecules, CD 45 (a molecular marker of bone marrow-derived cells) and macrophage-associated membrane molecules (CD 68, CD 14). Within the outflow tissue, the cells of the filtering trabecular meshwork stained with none of those reagents. However, infrequent single, dispersed, dendritic cells were positively stained in the intertrabecular spaces. More numerous labelled cells were found in the anterior- and posterior-most portions of the non-filtering part of the trabecular meshwork. These cells were continuous with stained cells adjacent to the outer wall of Schlemm's canal and to the collector channels. Numerous labelled cells were seen in the vicinity of the intra- and episcleral vessels, the ciliary meshwork, the stroma of the ciliary muscle and epithelial processes, and the iris stroma. With advancing age, increasing numbers of CD 45+, HLA class II expressing cells appeared to accumulate in the so-called uveoscleral pathway. These results indicate that bone marrow-derived cells with the potential to function of ACAID induction reside within human eyes, and that cells of this type are located not only in the stroma of iris and ciliary body, but within the non-filtering portions of the trabecular meshwork and the uveoscleral pathway. The appearance of rare CD 45+ cells "in transit" in the filtering trabecular meshwork is compatible with the view that cells carrying ACAID-inducing signals to the systemic immune apparatus escape from the eye by this route.
正常情况下占据小鼠虹膜和睫状体基质的骨髓源细胞与免疫现象——前房相关免疫偏离（ACAID）有关。向前房注射抗原后，这类细胞可能通过流出通道将诱导ACAID的信号传递到体循环中。为了在人类中识别此类细胞，对34只不同年龄组人类供体眼的前房进行免疫细胞化学染色，使用针对HLA II类分子、CD 45（骨髓源细胞的分子标志物）和巨噬细胞相关膜分子（CD 68、CD 14）的单克隆抗体。在流出组织中，滤过小梁网的细胞未被这些试剂染色。然而，在小梁间隙中偶尔可见单个、分散的树突状细胞呈阳性染色。在小梁网非滤过部分的最前部和最后部发现了更多被标记的细胞。这些细胞与Schlemm管外壁和集合管相邻的染色细胞相连。在巩膜内和巩膜上血管、睫状网、睫状肌基质和上皮突起以及虹膜基质附近可见大量被标记的细胞。随着年龄的增长，越来越多表达CD 45+、HLA II类分子的细胞似乎在所谓的葡萄膜巩膜途径中积聚。这些结果表明，具有诱导ACAID功能潜力的骨髓源细胞存在于人类眼睛中，并且这类细胞不仅位于虹膜和睫状体的基质中，还位于小梁网的非滤过部分和葡萄膜巩膜途径中。在滤过小梁网中罕见的“正在通过”的CD 45+细胞的出现与这样一种观点相符，即携带诱导ACAID信号的细胞通过这条途径从眼睛逃逸到全身免疫系统。
