[Experimental and clinical study on the chemosensitivity assay in vitro for head and neck tumor by AgNOR methods].

OBJECTIVE

To evaluate in vitro chemosensitivity of head and neck tumor to eight kinds of antineoplastic drugs by AgNOR technique.

METHOD

Fifty-nine cases' fresh specimens were obtained from head and neck tumors. We severed tumor tissue with simple mechanical separation method. After the acting of drugs, cell smear and argyophilie staining, mean Inhibiting rate was calculated. 12 cases were treated with chemotherapy at the same time.

RESULT

54 cases' specimens succeeded in the experiments, the rate of success was 91.5%. The changes of mean Inhibiting rate were from 0 to 63.0%. The chemosensitivity to DDP was the highest in eight kinds of antineoplastic drugs. VCR, 5-Fu, PYM were higher sensitive drugs respectively. The sensitivity to drugs in NPC was significantly higher than laryngeal cancer. The coincidence between the experiment and clinic was 75.0%.

CONCLUSION

AgNOR method is simple and convenient. In addition, it has clear correlation with the results of therapy. The method is useful for chemotherapy.