通过将精氨酸-甘氨酸-天冬氨酸细胞黏附信号嫁接到钙蛋白酶抑制蛋白片段来构建人工细胞黏附蛋白。

Engineering of artificial cell adhesion proteins by grafting the Arg-Gly-Asp cell adhesive signal to a calpastatin segment.

作者信息

Hashino K, Shimojo T, Kimizuka F, Kato I, Maeda T, Sekiguchi K, Titani K

机构信息

Biotechnology Research Laboratories, Takara Shuzo Co., Ltd., Shiga.

出版信息

J Biochem. 1992 Oct;112(4):547-51. doi: 10.1093/oxfordjournals.jbchem.a123936.

DOI:10.1093/oxfordjournals.jbchem.a123936

PMID:1283393

Abstract

A new artificial cell adhesive protein was engineered by grafting the Arg-Gly-Asp (RGD) sequence, the minimal recognition signal of fibronectin for interaction with integrins, to a calpastatin segment by in vitro mutagenesis. The mutagenized protein showed cell adhesive activity in addition to calpain inhibitory activity. The RGD signal grafted to the calpastatin segment was recognized by the vitronectin receptor but not by the fibronectin receptor.

摘要

通过体外诱变将纤连蛋白与整合素相互作用的最小识别信号精氨酸 - 甘氨酸 - 天冬氨酸（RGD）序列嫁接到钙蛋白酶抑制蛋白片段上，构建了一种新的人工细胞粘附蛋白。诱变后的蛋白除具有钙蛋白酶抑制活性外，还表现出细胞粘附活性。嫁接到钙蛋白酶抑制蛋白片段上的RGD信号可被玻连蛋白受体识别，但不能被纤连蛋白受体识别。