通过嫁接精氨酸-甘氨酸-天冬氨酸细胞黏附信号对细胞黏附性免疫球蛋白G进行工程改造。
Engineering of cell adhesive immunoglobulin G by grafting the Arg-Gly-Asp cell adhesive signal.
作者信息
Hashino K, Kato I, Kurosawa Y, Sekiguchi K, Titani K
机构信息
Biotechnology Research Laboratories, Takara Shuzo Co., Ltd., Shiga, Japan.
出版信息
Biosci Biotechnol Biochem. 1994 Jan;58(1):191-2. doi: 10.1271/bbb.58.191.
PMID:7764513
Abstract
A new artificial cell adhesive protein was engineered by grafting the Arg-Gly-Asp (RGD) sequence, the minimal recognition signal of fibronectin for interaction with integrin, to immunoglobulin G (IgG) by in vitro mutagenesis. The mutagenized protein showed cell adhesive activity on baby hamster kidney (BHK) cells.
摘要
通过体外诱变将纤连蛋白与整合素相互作用的最小识别信号——精氨酸-甘氨酸-天冬氨酸（RGD）序列嫁接到免疫球蛋白G（IgG）上，构建了一种新型人工细胞黏附蛋白。诱变后的蛋白在幼仓鼠肾（BHK）细胞上表现出细胞黏附活性。
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