Zhang Y, Mills G L, Nair M G
Bioactive Natural Products Laboratory, Department of Horticulture and National Food Safety and Toxicology Center Michigan State University, East Lansing, Michigan 48824, USA.
Phytomedicine. 2003;10(5):386-90. doi: 10.1078/0944-7113-00272.
In the search for bioactive natural products from edible mushrooms, we have investigated the fruiting body of Agrocybe aegerita. The methanol extract of this mushroom yielded a fatty acid fraction (FAF), along with palmitic acid (1), ergosterol (2), 5,8-epidioxy-ergosta-6,22-dien-3beta-ol (3), mannitol (4) and trehalose (5). The composition of FAF was confirmed by GC-MS and by comparison to the retention values of authentic samples of palmitic, stearic, oleic and linoleic acids. The structures of 1-5 were established using spectroscopic methods. FAF and compounds 1-3 showed cyclooxygenase (COX) enzyme inhibitory and antioxidant activities. The inhibition values of liposome peroxidation by FAF, compounds 1 and 2 at 100 microg/ml were 75, 45, and 43%, respectively. The inhibition values of COX-I enzyme by FAF and 1-3 at 100 microg/ml were 80, 39, 19, and 57%, respectively. Similarly, COX-II enzyme activity was reduced by FAF and 1-3 at 100 microg/ml with values of 88, 45, 28, and 22%, respectively. Compounds 1, 3 and fatty acids were isolated here for the first time from the fruiting body of A. aegerita.
在从食用菌中寻找生物活性天然产物的过程中，我们对柱状田头菇的子实体进行了研究。这种蘑菇的甲醇提取物产生了一个脂肪酸馏分（FAF），以及棕榈酸（1）、麦角甾醇（2）、5,8-环氧-麦角甾-6,22-二烯-3β-醇（3）、甘露醇（4）和海藻糖（5）。通过气相色谱-质谱联用（GC-MS）并与棕榈酸、硬脂酸、油酸和亚油酸标准样品的保留值进行比较，确定了FAF的组成。使用光谱方法确定了1-5的结构。FAF和化合物1-3表现出环氧化酶（COX）抑制活性和抗氧化活性。在100μg/ml时，FAF、化合物1和2对脂质体过氧化的抑制值分别为75%、45%和43%。在100μg/ml时，FAF和1-3对COX-I酶的抑制值分别为80%、39%、19%和57%。同样，在100μg/ml时，FAF和1-3使COX-II酶活性分别降低了88%、45%、28%和22%。化合物1、3和脂肪酸首次从柱状田头菇的子实体中分离得到。
