Akintunde A, Buxton D F
Department of Anatomy and Histology, College of Veterinary Medicine, Auburn University, AL 36849-5518.
J Neurosci Methods. 1992 Oct-Nov;45(1-2):15-22. doi: 10.1016/0165-0270(92)90039-g.
Four different fluorochromes were injected into adjacent cervical spinal cord segments, 1 unique tracer per segment. Each tracer, Fluoro-Gold, Fast Blue, Diamidino Yellow dihydrochloride and Propidium Iodide, was taken up by axonal terminals and transported intra-axonally in a retrograde direction to the cell bodies. Some, though by no means all, of these axons were stem axons with terminals in 2, 3 or 4 of the injected spinal segments. Hence as many as 4 different fluorescent tracers could be discerned simultaneously within individual neuronal somata of origin using fluorescent microscopy. These results extend the possibilities for multiple interconnection determinations within the central nervous system. Specifically, the potential for individual neurons of a nucleus to project collateral branches of a stem axon to as many as 4 different central nervous system nuclei now can be studied simultaneously using these 4 fluorescent tracers.
将四种不同的荧光染料注射到相邻的颈脊髓节段中，每个节段注射一种独特的示踪剂。每种示踪剂，即荧光金、快蓝、二盐酸双脒基黄和碘化丙啶，都被轴突终末摄取，并沿轴突逆行运输到细胞体。这些轴突中有些（但绝非全部）是主干轴突，其终末位于2个、3个或4个注射的脊髓节段中。因此，使用荧光显微镜可以在单个起源神经元胞体中同时辨别多达4种不同的荧光示踪剂。这些结果扩展了中枢神经系统内多种相互连接测定的可能性。具体而言，现在可以使用这4种荧光示踪剂同时研究一个核内的单个神经元将主干轴突的侧支投射到多达4个不同中枢神经系统核的可能性。
