Kawata Yoshikazu, Yano Shin-ichi, Kojima Hiroyuki
Special Division for Green Life Technology, National Institute of Advanced Industrial Science and Technology, 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan.
Biosci Biotechnol Biochem. 2003 May;67(5):1179-81. doi: 10.1271/bbb.67.1179.
Transformation of Escherichia coli is a basic technique for genetic engineering. We used a liposome-mediated lipofection method to transform electrocompetent E. coli cells which has little natural competence of foreign DNA without electroporation treatment, and got transformants with simple and quick treatment by a plasmid or a transposon and transposase complex.
大肠杆菌的转化是基因工程的一项基本技术。我们使用脂质体介导的脂质转染方法来转化电感受态大肠杆菌细胞，这种细胞在没有电穿孔处理的情况下对外源DNA几乎没有天然感受态，通过用质粒或转座子与转座酶复合物进行简单快速的处理即可获得转化体。
