Smith Allen A, Silver Timothy M
School of Graduate Medical Sciences, Barry University, Miami Shores, FL, USA.
J Cutan Pathol. 2003 Jul;30(6):401-4. doi: 10.1034/j.1600-0560.2003.00080.x.
Despite its occasional unavailability, alcian blue has long been the stain of choice for the pathognomonic mucin-secreting cells of extramammary Paget's disease. Zirconyl hematoxylin is a recently developed substitute for alcian blue, which is made from common laboratory materials.
We have stained sections from three different areas of the same case of extramammary Paget's disease with zirconyl hematoxylin, destained in 7% HCl in ethanol, restained with alcian blue at pH 2.5, destained in 2.5% trifluoroacetic acid in dichloromethane, and restained with zirconyl hematoxylin.
Each staining procedure stained the same cells.
Zirconyl hematoxylin is a useful alternative to alcian blue in the diagnosis of extramammary Paget's disease.
尽管阿尔辛蓝偶尔难以获取，但长期以来它一直是乳腺外佩吉特病中特征性黏液分泌细胞的首选染色剂。锆苏木精是一种最近开发的阿尔辛蓝替代品，由常见的实验室材料制成。
我们用锆苏木精对同一例乳腺外佩吉特病三个不同区域的切片进行染色，在乙醇中的7%盐酸中脱色，在pH 2.5的阿尔辛蓝中复染，在二氯甲烷中的2.5%三氟乙酸中脱色，然后用锆苏木精复染。
每种染色方法都能对相同的细胞进行染色。
在乳腺外佩吉特病的诊断中，锆苏木精是阿尔辛蓝的一种有用替代品。
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