Hu J Y, Ong S L, Ng W J, Lu F, Fan X J
Center for Water Research, Department of Civil Engineering, National University of Singapore, 10 Kent Ridge Crescent, 119260 Singapore.
Water Res. 2003 Aug;37(14):3463-71. doi: 10.1016/S0043-1354(03)00205-7.
This study investigated the characteristics of denitrifying phosphorus removal bacteria by using three different types of electron acceptors as well as the positive role of nitrite in phosphorus removal process. Denitrifying phosphorous removal bacteria was enriched under anaerobic-anoxic (A/A) condition. To understand A/A sludge better, sludge from two other sources were also studied. These include sludges obtained from a lab-scale anaerobic-anoxic-aerobic (A/A/O) system and a local sewage treatment plant. Three types of possible electron acceptors (oxygen, nitrate and nitrite) were examined for their roles in phosphorus uptake. The results obtained indicated that oxygen, nitrate and nitrite were able to act as electron acceptors successfully. This observation suggested that in addition to the two well-accepted groups of phosphorus removal bacteria (one can only utilize oxygen to take up phosphorus, P(O), while the other can use both oxygen and nitrate, P(ON)), a new group of phosphorus removal bacteria, P(ON(n)), which could use oxygen, nitrate or nitrite to take up phosphorus was identified. The relative population of these three types of bacteria could be calculated from results obtainable from phosphorus uptake batch experiments with either oxygen or nitrate or nitrite as electron acceptor. The results obtained in this study showed that A/A sludge had similar phosphorus removal performance as the A/A/O sludge. However, it has better denitrifying phosphorus removal capability, which was demonstrated by the relative population of the three groups of bacteria. The results also suggested that nitrite was not an inhibitor to phosphorus removal process. Instead, it is an alternative electron acceptor to oxygen or nitrate.
本研究通过使用三种不同类型的电子受体来研究反硝化除磷细菌的特性，以及亚硝酸盐在除磷过程中的积极作用。在厌氧-缺氧（A/A）条件下富集反硝化除磷细菌。为了更好地了解A/A污泥，还研究了来自其他两个来源的污泥。这些污泥包括从实验室规模的厌氧-缺氧-好氧（A/A/O）系统和当地污水处理厂获得的污泥。研究了三种可能的电子受体（氧气、硝酸盐和亚硝酸盐）在磷吸收中的作用。所得结果表明，氧气、硝酸盐和亚硝酸盐能够成功地作为电子受体。这一观察结果表明，除了两组公认的除磷细菌（一组只能利用氧气吸收磷，即P（O），而另一组可以利用氧气和硝酸盐，即P（ON））之外，还鉴定出了一组新的除磷细菌，即P（ON（n）），它可以利用氧气、硝酸盐或亚硝酸盐来吸收磷。这三种类型细菌的相对数量可以通过以氧气或硝酸盐或亚硝酸盐作为电子受体的磷吸收批次实验获得的结果来计算。本研究获得的结果表明，A/A污泥与A/A/O污泥具有相似的除磷性能。然而，它具有更好的反硝化除磷能力，这通过三组细菌的相对数量得到了证明。结果还表明，亚硝酸盐不是除磷过程的抑制剂。相反，它是氧气或硝酸盐的替代电子受体。
