Zimmer J L, Slawson R M, Huck P M
Department of Biology, University of Waterloo, Waterloo, Ont., N2L 3G1, Canada.
Water Res. 2003 Aug;37(14):3517-23. doi: 10.1016/S0043-1354(03)00238-0.
This study investigated the level of inactivation and the potential for Cryptosporidium parvum to repair following low doses (1 and 3mJ/cm(2)) of ultraviolet (UV) irradiation from both low- and medium-pressure UV lamps. Cryptosporidium parvum oocysts suspended in phosphate buffered saline were exposed to UV using a bench-scale collimated beam apparatus. Oocyst suspensions were incubated at 5 degrees C or 25 degrees C under light and dark conditions up to 120 h (5 days) following exposure to UV irradiation, to examine photoreactivation and dark repair potential, respectively. Cryptosporidium parvum infectivity was determined throughout the incubation period using an HCT-8 cell culture and an antibody staining procedure for detection. No detectable evidence of repair was observed after incubation under light or dark conditions following either LP or MP UV lamp irradiation.
本研究调查了低剂量（1和3mJ/cm²）的低压和中压紫外线照射后微小隐孢子虫的失活水平及其修复潜力。将悬浮于磷酸盐缓冲盐水中的微小隐孢子虫卵囊，使用台式准直光束装置进行紫外线照射。卵囊悬液在紫外线照射后，分别于5℃或25℃的光照和黑暗条件下孵育长达120小时（5天），以分别检测光复活和暗修复潜力。在整个孵育期间，使用HCT-8细胞培养和抗体染色程序检测微小隐孢子虫的感染性。在低压或中压紫外线灯照射后，无论是在光照还是黑暗条件下孵育，均未观察到可检测到的修复证据。
