Moussaoui S M, Hermans E, Mathieu A M, Bonici B, Clerc F, Guinet F, Garret C, Laduron P M
Rhône-Poulenc Rorer, Research Center, Vitry sur Seine, France.
Neuroreport. 1992 Dec;3(12):1073-6. doi: 10.1097/00001756-199212000-00010.
We have developed antibodies against the NK1 receptor and have investigated its cellular distribution. Rabbit polyclonal antibodies were generated against peptide (19-32) of the rat brain NK1 receptor. They were very specific to the NK1 site as shown by ELISA against various epitopes of NK1, NK2 and NK3 receptors and by immunoblotting of proteins from bacteria transfected with rat brain NK1 receptor cDNA and from rat cortex. Determining how immunostained NK1 receptors are distributed in the rat spinal cord made it possible to identify the cellular structures on which NK1 receptors are located and where they form synapses with SP terminals. In the superficial layers of the dorsal horn, the NK1 receptors appeared mainly of dendritic nature and were, like SP, abundant. In the deep layers of the dorsal horn and in the ventral horn, they were associated mostly with cell bodies.
我们已开发出针对NK1受体的抗体，并研究了其细胞分布。针对大鼠脑NK1受体的肽段（19 - 32）制备了兔多克隆抗体。通过针对NK1、NK2和NK3受体各种表位的ELISA以及对用大鼠脑NK1受体cDNA转染的细菌和大鼠皮层中的蛋白质进行免疫印迹分析，结果表明这些抗体对NK1位点具有高度特异性。确定免疫染色的NK1受体在大鼠脊髓中的分布情况，有助于识别NK1受体所在的细胞结构以及它们与P物质（SP）终末形成突触的位置。在背角浅层，NK1受体主要呈现树突性质，并且与P物质一样丰富。在背角深层和腹角，它们大多与细胞体相关联。
