口面部疼痛会增加大鼠三叉神经尾侧核中甘丙肽的mRNA水平。

Orofacial pain increases mRNA level for galanin in the trigeminal nucleus caudalis of the rat.

作者信息

Tokunaga A, Senba E, Manabe Y, Shida T, Ueda Y, Tohyama M

机构信息

Department of Anesthesiology, Osaka Dental University, Japan.

出版信息

Peptides. 1992 Nov-Dec;13(6):1067-72. doi: 10.1016/0196-9781(92)90007-p.

DOI:10.1016/0196-9781(92)90007-p

PMID:1283628

Abstract

The changes of preprogalanin mRNA levels in the superficial dorsal horn neurons (laminae I and II) of the trigeminal nucleus caudalis in response to orofacial pain induced by the injection of 5% formalin into the lips of rats was investigated and compared to those of preproenkephalin A mRNA and preprodynorphin mRNA in the same region by means of in situ hybridization histochemistry. Rapid and marked increases of preprogalanin and preprodynorphin mRNA were observed on the side of the injection, but the increase of preproenkephalin A mRNA level was less pronounced than that of the other two mRNAs, indicating that these peptides have different roles in the dorsal horn analgesic mechanism and that galanin, in addition to opioid peptides, may have a highly specific role in this mechanism.

摘要

研究了向大鼠唇部注射5%福尔马林诱导口面部疼痛时，三叉神经尾核浅层背角神经元（I层和II层）中前甘丙肽原mRNA水平的变化，并通过原位杂交组织化学方法，将其与同一区域的前脑啡肽原A mRNA和前强啡肽原mRNA的变化进行比较。在注射侧观察到前甘丙肽原和前强啡肽原mRNA迅速且显著增加，但前脑啡肽原A mRNA水平的增加不如其他两种mRNA明显，这表明这些肽在背角镇痛机制中具有不同作用，并且甘丙肽除了阿片肽外，可能在该机制中具有高度特异性作用。