Holmes P V, Blanchard D C, Blanchard R J, Brady L S, Crawley J N
Section on Behavioral Neuropharmacology, National Institute of Mental Health, Bethesda, MD 20892, USA.
Pharmacol Biochem Behav. 1995 Apr;50(4):655-60. doi: 10.1016/0091-3057(94)00334-3.
Galanin is a 29 amino acid neuropeptide that coexists with norepinephrine in approximately 80% of locus coeruleus (LC) neurons in the rat. The effects of chronic, naturalistic stress on preprogalanin mRNA in the LC was studied. The visible burrow system (VBS) served as the stress paradigm. Long-Evans rats (three males and two females) were housed together in the VBS for 13 days. The males established dominance hierarchies during this period. On day 14, preprogalanin mRNA in the LC was significantly elevated in subordinate rats compared to dominant and control rats, as measured by quantitative in situ hybridization. Levels of mRNA were positively correlated with the number of wounds by day 7 and day 14 and negatively correlated with body weight gain by day 14. These results suggest that the neuropeptide galanin may be overexpressed during chronic stress in rats.
甘丙肽是一种由29个氨基酸组成的神经肽，在大鼠中，约80%的蓝斑（LC）神经元中与去甲肾上腺素共存。研究了慢性、自然应激对LC中前甘丙肽原mRNA的影响。可见洞穴系统（VBS）作为应激模型。将Long-Evans大鼠（3只雄性和2只雌性）一起饲养在VBS中13天。在此期间，雄性大鼠建立了优势等级制度。在第14天，通过定量原位杂交测量，与优势大鼠和对照大鼠相比，从属大鼠LC中的前甘丙肽原mRNA显著升高。到第7天和第14天，mRNA水平与伤口数量呈正相关，到第14天与体重增加呈负相关。这些结果表明，神经肽甘丙肽可能在大鼠慢性应激期间过度表达。
