雌二醇和生长激素释放激素对体外培养垂体细胞甘丙肽分泌的调节

Regulation of galanin secretion from pituitary cells in vitro by estradiol and GHRH.

作者信息

Hemmer A, Hyde J F

机构信息

Department of Anatomy and Neurobiology, University of Kentucky College of Medicine, Lexington 40536.

出版信息

Peptides. 1992 Nov-Dec;13(6):1201-6. doi: 10.1016/0196-9781(92)90029-3.

DOI:10.1016/0196-9781(92)90029-3

PMID:1283631

Abstract

The effects of estradiol and growth hormone-releasing hormone (GHRH) on galanin release from anterior pituitary cells were examined in vitro. 17-beta-Estradiol (0.001-10 nM) increased galanin secretion from anterior pituitary cells in a concentration-dependent manner. Estradiol (10 nM) increased galanin release 300 and 600% from pituitary cells of ovariectomized and male rats, respectively. Immunocytochemical studies demonstrated that estradiol (10 nM) increased the number of galanin-containing cells twofold after 4 days in culture. Growth hormone-releasing hormone (1 and 10 nM) increased and SRIF (1 and 10 nM) decreased galanin release from pituitary cells of ovariectomized and male rats. We conclude that estradiol increases galanin release by a direct effect on pituitary cells, in part by increasing the number of pituitary cells synthesizing galanin. In addition, GHRH stimulates galanin release when estradiol levels are low.

摘要

在体外研究了雌二醇和生长激素释放激素（GHRH）对垂体前叶细胞甘丙肽释放的影响。17-β-雌二醇（0.001 - 10 nM）以浓度依赖的方式增加垂体前叶细胞的甘丙肽分泌。雌二醇（10 nM）使去卵巢大鼠和雄性大鼠垂体细胞的甘丙肽释放分别增加300%和600%。免疫细胞化学研究表明，培养4天后，雌二醇（10 nM）使含甘丙肽细胞的数量增加了两倍。生长激素释放激素（1和10 nM）增加，而生长抑素（1和10 nM）减少去卵巢大鼠和雄性大鼠垂体细胞的甘丙肽释放。我们得出结论，雌二醇通过对垂体细胞的直接作用增加甘丙肽释放，部分是通过增加合成甘丙肽的垂体细胞数量。此外，当雌二醇水平较低时，GHRH刺激甘丙肽释放。