Hori T, Guo F, Tanaka Y, Uesugi S
Department of Environment and Natural Sciences, Graduate School of Environment and Information Sciences, Yokohama National University, 79-7 Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan.
Nucleic Acids Res Suppl. 2001(1):201-2. doi: 10.1093/nass/1.1.201.
We constructed a new type of trans-acting HDV ribozyme which is based on the antigenomic RNA sequence and has an additional binding site to form an extra stem (P5) of 7 base-pairs introduced in the J1/2 region between P1 and P2 stems. A substrate RNA containing the two binding sequences was specifically cleaved while no selectivity was observed in the case of the wild-type ribozyme with only one binding site. Mutation to produce two mismatch base-pairs in the central part of the P5 stem abolished the specific cleavage.
我们构建了一种新型的反式作用丁型肝炎病毒核酶，它基于反基因组RNA序列，并且有一个额外的结合位点，可在P1和P2茎之间的J1/2区域形成一个由7个碱基对组成的额外茎（P5）。含有这两个结合序列的底物RNA被特异性切割，而在只有一个结合位点的野生型核酶的情况下未观察到选择性。在P5茎中部产生两个错配碱基对的突变消除了特异性切割。
