[Effect of oxidated low-density lipoprotein on early apoptosis of vascular smooth muscle cell].

OBJECTIVE

To investigate the apoptosis in vascular smooth muscle cell (VSMC) induced by oxidated low-density lipoprotein cholesterol (ox-LDL), and to discuss the relative mechanism of the apoptosis of VSMC.

METHODS

The growth index of VSMC of Wistar rat was determined. By using trypan blue to count sustaining cells, the proliferative effect of ox-LDL on culturing VSMC was observed. With flow cytometer, the apoptosis of VSMC induced by ox-LDL and the inhibitory effect of cyclooxygenase-2 (COX-2) inhibitor NS-398 on apoptosis were determined.

RESULTS

After VSMC grew normally in culture, ox-LDL in concentrations of 35 mg/L and 50 mg/L could advance the growth period of VSMC to between 12 and 24 hours, and the proliferation of VSMC were obvious. The proliferation of VSMC induced by ox-LDL were inhibited by NS-398. The rate of apoptosis of VSMC in ox-LDL and NS-398 groups were higher than that of normal control.

CONCLUSION

ox-LDL could induce an obvious proliferation of VSMC in culture. Although NS-398 can decrease the proliferation of VSMC, it could enhance ox-LDL to induce apoptosis.