Cavuşoğlu Cengiz, Bilgiç Altinay
Ege Universitesi Tip Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, Izmir.
Mikrobiyol Bul. 2003 Jan;37(1):13-8.
Rifampin is one of the most potent antituberculosis drugs and therefore, rifampin resistance leads to high clinical relapse rates. Detection of rifampin resistance could be an indication of multidrug resistance. In the recent years several molecular methods have been developed to evaluate the mutations in rpoB gene for the detection of rifampin resistance. The aim of the present study was to evaluate the performance of the RNA/RNA Mismatch Assay for detection of the mutations in the rpoB gene in 20 M. tuberculosis isolates which were determined as resistant to rifampin by agar proportion method. While RNA/RNA Mismatch Assay detected the mutations in the rpoB region in 16 of 20 (80%) M. tuberculosis isolates, the remaining four isolates yielded no band pattern indicating resistance. However, there may be situations where interpretation of the results is difficult in RNA/RNA Mismatch Assay which is already cheaper than DNA sequencing and other molecular methods. In conclusion, if the RNA/RNA Mismatch Assay can be optimized, it can be used for the rapid detection of rifampin resistance.
利福平是最有效的抗结核药物之一，因此，利福平耐药会导致较高的临床复发率。利福平耐药的检测可能提示多重耐药。近年来，已开发出多种分子方法来评估rpoB基因中的突变，以检测利福平耐药性。本研究的目的是评估RNA/RNA错配分析检测20株经琼脂比例法确定对利福平耐药的结核分枝杆菌分离株中rpoB基因突变的性能。RNA/RNA错配分析在20株结核分枝杆菌分离株中的16株（80%）检测到了rpoB区域的突变，其余4株未产生表明耐药的条带模式。然而，在RNA/RNA错配分析中，可能存在结果解释困难的情况，而该分析已比DNA测序和其他分子方法便宜。总之，如果RNA/RNA错配分析能够优化，它可用于利福平耐药性的快速检测。
