• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

[Construction of the eukaryotic expression vector PsecTaq2A-AMG for human amelogenin].

作者信息

Yang Ailing, Xu Chenrong, Zhang Jincai

机构信息

Oral Medicine of West China College of Stomatology, Sichuan University, Chengdu 610041, China.

出版信息

Hua Xi Kou Qiang Yi Xue Za Zhi. 2003 Apr 20;21(2):133-5.

PMID:12838701
Abstract

OBJECTIVE

The purpose of this study was to construct a eukaryotic expression vector for human amelogenin (AMG).

METHODS

PCR was performed to amplify the AMG encoding region. Amplified fragments for human AMG were recovered and inserted into eukaryotic expression vectors PsecTaq2A. The recombinant plasmid PsecTaq2A-AMG was constructed and their positive clones were identified.

RESULTS

  1. Amplified products were checked by electrophoresis and the results were satisfactory. 2. The recombinant plasmid PsecTaq2A-AMG was analyzed by restriction endonuclease mapping and DNA sequencing. The results of sequencing were consistent with those from GenBank.

CONCLUSION

The recombinant plasmid PsecTaq2A-AMG was successfully constructed with properly inserted DNA sequence encoding mature amelogenin.

摘要

相似文献

1
[Construction of the eukaryotic expression vector PsecTaq2A-AMG for human amelogenin].
Hua Xi Kou Qiang Yi Xue Za Zhi. 2003 Apr 20;21(2):133-5.
2
[Construction of eukaryotic expression clone for human amelogenin].[人釉原蛋白真核表达克隆的构建]
Sichuan Da Xue Xue Bao Yi Xue Ban. 2004 Jan;35(1):8-10.
3
[Cloning of human amelogenin gene encoding mature peptide].[编码成熟肽的人釉原蛋白基因的克隆]
Shanghai Kou Qiang Yi Xue. 2004 Apr;13(2):126-9.
4
Construction and identification of eukaryotic expression vector of human full-length PLCgamma1 gene.人全长PLCγ1基因真核表达载体的构建与鉴定
Di Yi Jun Yi Da Xue Xue Bao. 2004 Aug;24(8):849-53.
5
[Construction of eukaryote expression vector carrying human soluble interleukin-1 receptor gene].
Hua Xi Kou Qiang Yi Xue Za Zhi. 2003 Apr 20;21(2):140-3.
6
[Expression of rat amelogenin gene in Escherichia coli].[大鼠釉原蛋白基因在大肠杆菌中的表达]
Zhonghua Kou Qiang Yi Xue Za Zhi. 2001 Jan;36(1):45-7.
7
Construction of a eukaryotic expression plasmid of Humanin.人胰岛素原真核表达质粒的构建。 需要说明的是，你原文中的“Humanin”一般译为“人胰岛素原”，但感觉你这里可能有误，通常构建表达的是“Humanin”（人胰岛素），如果是这样，正确译文是：人胰岛素真核表达质粒的构建。
J Zhejiang Univ Sci B. 2005 Jan;6(1):11-3. doi: 10.1631/jzus.2005.B0011.
8
[Construction of recombinant eukaryotic expression plasmid pcDNA3.1(+)-mtDNA of human colorectal carcinoma cells].[人结肠癌细胞重组真核表达质粒pcDNA3.1(+)-mtDNA的构建]
Di Yi Jun Yi Da Xue Xue Bao. 2005 Aug;25(8):1016-9.
9
[Construction of LACK gene recombinant plasmid and detection of its expression in eukaryotic cell].
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2004 Apr;21(2):272-5.
10
[Construction and expression of human alpha-fetoprotein recombinant plasmid].[人甲胎蛋白重组质粒的构建与表达]
Sichuan Da Xue Xue Bao Yi Xue Ban. 2004 Sep;35(5):612-4, 633.