博来霉素对DNA序列特异性切割的起源
Origin of sequence specific cleavage of DNA by bleomycins.
作者信息
Kuroda R, Shinomiya M, Otsuka M
机构信息
Department of Chemistry, College of Arts and Sciences, University of Tokyo, Japan.
出版信息
Nucleic Acids Symp Ser. 1992(27):9-10.
A new DNA photocleaving agent which contains bleomycin A2's DNA binding portion and its mono- and terthiazole analogues have been designed and synthesized, and their DNA binding mode and cleavage base specificity have been studied. The photoactive p-nitrobenzoyl group attached at the end of molecules cleaves DNA on UV irradiation. All the oligo-thiazole compounds exhibited high sequence specificity in DNA scission. The bithiazole derivative did not cleave DNA at or near 5'-GpT-3' or 5'-GpC-3' as expected from widely believed DNA binding mechanism of the antibiotics.
一种新型DNA光裂解剂已被设计并合成，它含有博来霉素A2的DNA结合部分及其单噻唑和三噻唑类似物，并且对它们的DNA结合模式和切割碱基特异性进行了研究。连接在分子末端的光活性对硝基苯甲酰基在紫外线照射下可切割DNA。所有的寡噻唑化合物在DNA断裂中都表现出高度的序列特异性。双噻唑衍生物并未如人们广泛认为的抗生素DNA结合机制所预期的那样，在5'-GpT-3'或5'-GpC-3'处或其附近切割DNA。
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