Mistry J S, Koepsel R R, Lazo J S
Department of Pharmacology, University of Pittsburgh, School of Medicine, PA 15261.
Biochem Biophys Res Commun. 1993 Mar 15;191(2):420-6. doi: 10.1006/bbrc.1993.1234.
DNA strand scission by the bleomycin analogs talisomycin S10b and a novel fluorescent mimic, fluoromycin, has been characterized and compared with that of bleomycin A2. Both analogs were found to have essentially the same sequence specificity for DNA strand scission as bleomycin A2. As observed with bleomycin A2, the preferred sites of DNA cleavage by talisomycin S10b and fluoromycin were 5'-GpT-3' and 5'-GpC-3'. These results suggest that the DNA sequence selectivity of bleomycins remains unaltered upon modification of the C-terminal domain.
博来霉素类似物他利霉素S10b和一种新型荧光模拟物氟霉素对DNA链的断裂作用已得到表征，并与博来霉素A2进行了比较。发现这两种类似物对DNA链断裂的序列特异性与博来霉素A2基本相同。正如在博来霉素A2中观察到的那样，他利霉素S10b和氟霉素切割DNA的优选位点是5'-GpT-3'和5'-GpC-3'。这些结果表明，对博来霉素C末端结构域进行修饰后，其DNA序列选择性保持不变。
