一类新表位在结直肠癌、相邻及远处黏膜的小肠黏蛋白上的表达。

Expression of a novel family of epitopes on small intestinal mucins in colorectal cancers, adjacent and remote mucosa.

作者信息

Pilbrow S J, Hertzog P J, Pinczower G D, Linnane A W

机构信息

Biochemistry Department, Monash University, Clayton, Vic., Australia.

出版信息

Tumour Biol. 1992;13(5-6):251-67. doi: 10.1159/000217774.

DOI:10.1159/000217774

PMID:1283925

Abstract

We describe the production, immunochemical and immunohistochemical characterization of monoclonal antibodies (MAbs) raised against the oncofetal small intestinal mucin antigen (SIMA). Four MAbs, reacting with distinct neuraminidase-sensitive SIMA epitopes, were shown to define a novel differentiation-associated relationship of SIMA epitopes within the normal small intestinal villus. Using Swiss rolls of 15 entire colorectal cancer resections, inappropriate expression of SIMA epitopes was detected in all cancers, in adjacent transitional mucosa and remote morphologically normal mucosa, extending as far as resection margins (73%). SIMA expression, whether preexisting or reactive to the tumor, may predispose to malignant change and tumor recurrence.

摘要

我们描述了针对癌胚性小肠粘蛋白抗原（SIMA）产生的单克隆抗体（MAb）的制备、免疫化学和免疫组织化学特征。四种与不同的神经氨酸酶敏感SIMA表位发生反应的单克隆抗体，被证明可确定正常小肠绒毛内SIMA表位一种新的分化相关关系。利用15例完整结直肠癌切除术的瑞士卷标本，在所有癌症、相邻的移行黏膜以及远处形态学正常的黏膜中均检测到SIMA表位的异常表达，其范围延伸至手术切缘（73%）。SIMA表达，无论是预先存在的还是对肿瘤有反应的，都可能易导致恶性改变和肿瘤复发。