Center for Infectious Diseases and Vaccinology, Arizona State University, Tempe, AZ 85287, USA.
Nat Rev Microbiol. 2010 Nov;8(11):791-801. doi: 10.1038/nrmicro2423.
Appropriately simulating the three-dimensional (3D) environment in which tissues normally develop and function is crucial for engineering in vitro models that can be used for the meaningful dissection of host-pathogen interactions. This Review highlights how the rotating wall vessel bioreactor has been used to establish 3D hierarchical models that range in complexity from a single cell type to multicellular co-culture models that recapitulate the 3D architecture of tissues observed in vivo. The application of these models to the study of infectious diseases is discussed.
适当地模拟组织正常发育和功能的三维（3D）环境对于工程体外模型至关重要，这些模型可用于对宿主-病原体相互作用进行有意义的剖析。本综述强调了旋转壁式生物反应器如何用于建立 3D 层次模型，这些模型的复杂性范围从单个细胞类型到再现体内观察到的组织 3D 结构的多细胞共培养模型。还讨论了这些模型在传染病研究中的应用。
