Xu Shi-jie, Wang Jian-xin, Yang Dong-ping
Department of Oncology, The First Affilicated Hospital of Jiaxing Medical College, Jiaxing University, Zhejiang 314000, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2003 Mar;37(2):119-20.
To evaluate their genotoxic risk in nurses occupationally exposed to antineoplastic drugs with chromosomal aberration test and cytokinesis-block micronucleus (CBMN) test.
Sixteen nurses in the exposure group were selected from the oncology department of the same hospital, with an average length of exposure of 5.5 years and daily making 8.25 chemotherapeutic preparations in average. The controls were students from a nursing school. Peripheral blood from both groups was cultured at 37 degrees C for 48 h and 72 h, respectively, and then slides were prepared for conventional chromosomal aberration test and CBMN test. One hundred blood cells in metaphase and 1 000 binuclear lymphocytes in each sample were observed under microscope.
The results showed that the mean chromosomal aberration rate in the exposure group was (6.38 +/- 3.30)%, significantly higher than that in the controls (1.25 +/- 0.93)% (P < 0.01). And, the mean micronucleated cell rate in the exposure group was (15.06 +/- 5.30) per thousand, very significantly higher than that in the controls (4.56 +/- 1.67) per thousand (P < 0.01).
The investigation indicated that chromosome damage rate in the nurses from oncology department was higher than that in the controls, which may be related to their occupational exposure to antineoplastic drugs.
通过染色体畸变试验和胞质分裂阻滞微核（CBMN）试验评估职业接触抗肿瘤药物的护士的遗传毒性风险。
从同一家医院的肿瘤科选取16名暴露组护士，平均暴露年限为5.5年，平均每天配制8.25种化疗制剂。对照组为某护理学校的学生。两组的外周血分别在37℃培养48小时和72小时，然后制备玻片进行常规染色体畸变试验和CBMN试验。在显微镜下观察每个样本中的100个中期血细胞和1000个双核淋巴细胞。
结果显示，暴露组的平均染色体畸变率为（6.38±3.30）%，显著高于对照组（1.25±0.93）%（P<0.01）。并且，暴露组的平均微核细胞率为千分之（15.06±5.30），极显著高于对照组千分之（4.56±1.67）（P<0.01）。
调查表明，肿瘤科护士的染色体损伤率高于对照组，这可能与其职业接触抗肿瘤药物有关。
