[软组织平滑肌肉瘤中3p染色体位点杂合性的特异性缺失]
[Specific loss of heterozygosity of chromosome 3p loci in soft tissue leiomyosarcoma].
作者信息
Peng Hui, Yang Guang-hua, Bu Hong, Gou Li-xin
机构信息
Department of Pathology, Huaxi Hospital, Sichuan University, Chengdu 610041, China.
出版信息
Zhonghua Bing Li Xue Za Zhi. 2003 Apr;32(2):124-7.
OBJECTIVE
To analyze the loss of heterozygosity (LOH) at 5 loci on chromosome 3p in soft tissue leiomyosarcoma (LMS).
METHODS
LOH was detected in 22 cases of LMS using PCR-silver staining targeting 5 microsatellite sites on 3p14.2-pter. Relation between LOH and LMS clinical pathological features was also analyzed.
RESULTS
Ten of 22 LMS samples showed LOH at more than one locus (45.4%). Among the 5 loci, LOH occurred more frequently at D3s1295 (36.8%) and D3s1289 (10.5%), but absent at D3s1293. No significant difference was found on LOH incidence between different grade, size and location of LMS.
CONCLUSIONS
LOH on chromosome 3p14.2-23 region is relatively frequent in LMS. Region around D3s1295 and D3s1289 may harbor tumor suppressor gene relating to LMS.
目的
分析软组织平滑肌肉瘤（LMS）中3号染色体短臂上5个位点的杂合性缺失（LOH）情况。
方法
采用针对3p14.2 - pter上5个微卫星位点的PCR - 银染法，检测22例LMS的LOH情况。同时分析LOH与LMS临床病理特征之间的关系。
结果
22例LMS样本中有10例在一个以上位点出现LOH（45.4%）。在5个位点中，D3s1295（36.8%）和D3s1289（10.5%）的LOH发生率较高，而D3s1293未出现LOH。不同分级、大小和部位的LMS在LOH发生率上未发现显著差异。
结论
3p14.2 - 23区域的LOH在LMS中相对常见。D3s1295和D3s1289周围区域可能存在与LMS相关的肿瘤抑制基因。
Zotero 插件