Tang Xuan-li, Yao Gen-you, Chen Li-rong, Yang Ze-ran, Li Shi-lan
Department of Pathology, Zhejiang University School of Medical, Hangzhou 310031, China.
Zhonghua Wai Ke Za Zhi. 2006 Oct 1;44(19):1314-7.
To study the loss of heterozygosity (LOH) on chromosome 3p in breast cancers and precancerous lesion.
LOH at 11 microsatellite loci was detected in 41 cases of breast cancers and 12 cases of precancerous lesion by polymerase chain reaction and silver stain. The expressions of ER, PR, FHIT and hMLH1 were detected in breast cancer by immunohistochemistry.
LOH on 3p was detected in 97% of breast cancers. D3S1295, D3S1029 and D3S1038 located at 3p14, 3p21-p22 and 3p25 were identified as the loci with most frequent LOH (53.1%, 43.6% and 52.5%). LOH of D3S1038 and expression of hMLH1 protein correlated with several clinicopathological features. LOH of D3S1295 had significant negative correlation with the expression of FHIT. In breast precancerous lesions, LOH on 3p was detected in 41.7% lesions. D3S1295 and D3S1029 were also identified as the most frequent LOH locus (27.3% and 16.7%). The smallest common LOH region seems likely lie between 3p14 and 3p25.
The smallest LOH region indicates the existence of breast tumor related genes and some of them affect gene expression.
研究乳腺癌及癌前病变中3号染色体短臂（3p）杂合性缺失（LOH）情况。
采用聚合酶链反应和银染法检测41例乳腺癌及12例癌前病变中11个微卫星位点的LOH。采用免疫组织化学法检测乳腺癌中雌激素受体（ER）、孕激素受体（PR）、脆性组氨酸三联体基因（FHIT）及错配修复蛋白（hMLH1）的表达。
97%的乳腺癌检测到3p的LOH。位于3p14的D3S1295、3p21 - p22的D3S1029及3p25的D3S1038被确定为LOH最常见的位点（分别为53.1%、43.6%和52.5%）。D3S1038的LOH及hMLH1蛋白表达与多项临床病理特征相关。D3S1295的LOH与FHIT表达呈显著负相关。在乳腺癌前病变中，41.7%的病变检测到3p的LOH。D3S1295和D3S1029也被确定为最常见的LOH位点（分别为27.3%和16.7%）。最小的共同LOH区域似乎位于3p14和3p25之间。
最小的LOH区域提示存在与乳腺肿瘤相关的基因，其中一些基因影响基因表达。
