Ponniah Gomathinayagam, Chen Han, Michielutti Ronda, Salonen Nancy, Blum Paul
School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.
Appl Environ Microbiol. 2003 Jul;69(7):4227-35. doi: 10.1128/AEM.69.7.4227-4235.2003.
The efficiency of enterobacterial disinfection is dependent largely on enterobacterial community physiology. However, the relationship between enterobacterial community physiology and wastewater processing is unclear. The purpose of this study was to investigate this relationship. The influence of wastewater treatment processes on enterobacterial community physiology was examined at the single-cell level by using culture-independent methods. Intracellular concentrations of two conserved proteins, the growth-related protein Fis and the stationary-phase protein Dps, were analyzed by epifluoresence microscopy of uncultivated cells by using enterobacterial group-specific polyclonal fluorochrome-coupled antibodies. Enterobacterial single-cell community protein profiles were distinct for different types of biological treatment. The differences were not apparent when bulk methods of protein analysis were used. Trickling filter wastewater yielded Fis-enriched communities compared to the communities in submerged aeration basin wastewater. Community differences in Fis and Dps contents were used to predict disinfection efficiency. Disinfection of community samples by heat exposure combined with cultivation in selective media confirmed that enterobacterial communities exhibited significant differences in sensitivity to disinfection. These findings provide strategies that can be used to increase treatment plant performance, reduce the enterobacterial content in municipal wastewater, and minimize the release of disinfection by-products into receiving water.
肠道细菌消毒的效率在很大程度上取决于肠道细菌群落的生理状态。然而，肠道细菌群落生理状态与废水处理之间的关系尚不清楚。本研究的目的是调查这种关系。通过使用非培养方法，在单细胞水平上研究了废水处理过程对肠道细菌群落生理状态的影响。利用肠道细菌群特异性多克隆荧光染料偶联抗体，通过未培养细胞的落射荧光显微镜分析了两种保守蛋白（生长相关蛋白Fis和稳定期蛋白Dps）的细胞内浓度。不同类型的生物处理中，肠道细菌单细胞群落的蛋白质谱各不相同。当使用蛋白质分析的整体方法时，这些差异并不明显。与淹没曝气池废水中的群落相比，滴滤池废水产生了富含Fis的群落。利用Fis和Dps含量的群落差异来预测消毒效率。通过热暴露结合在选择性培养基中培养对群落样本进行消毒，证实肠道细菌群落在对消毒的敏感性上存在显著差异。这些发现提供了可用于提高处理厂性能、降低城市废水中肠道细菌含量以及减少消毒副产物向受纳水体排放的策略。
