使用酶联免疫吸附测定法在微孔中进行非放射性杂交以检测逆转录聚合酶链反应扩增的细胞角蛋白19和癌胚抗原信使核糖核酸
Non-radioactive hybridization in microwells using enzyme linked immune sorbent assay for detection of RT-PCR-amplified CK19- and CEA-mRNA.
作者信息
Zhong X, Kaul S, Eichler A, Bastert G
机构信息
Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical University, Wuhan 430030.
出版信息
J Tongji Med Univ. 1999;19(3):181-4, 189. doi: 10.1007/BF02887729.
PCR enzyme linked immune sorbent assay (ELISA) was developed for detection of RT-PCR-amplified cytokeratin 19 (CK19) mRNA and carcinoembryonic antigen (CEA) mRNA. The non-radioactive hybridization was performed in a streptavidin-coated microwell with digoxigenin-labeled PCR products and with biotin-labeled capture probe. PCR ELISA was proved to be expedient, simple, sensitive and safe for identification of CK19-, CEA-RT-PCR products. These results were proven by sequencing.
聚合酶链反应酶联免疫吸附测定（ELISA）被开发用于检测逆转录聚合酶链反应（RT-PCR）扩增的细胞角蛋白19（CK19）信使核糖核酸（mRNA）和癌胚抗原（CEA）mRNA。非放射性杂交在链霉抗生物素蛋白包被的微孔中进行，使用地高辛标记的PCR产物和生物素标记的捕获探针。PCR ELISA被证明对于鉴定CK19 -、CEA - RT-PCR产物是方便、简单、灵敏且安全的。这些结果通过测序得到证实。
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