肺炎链球菌的三种细胞外锌金属蛋白酶对小鼠毒力的影响不同。
The three extra-cellular zinc metalloproteinases of Streptococcus pneumoniae have a different impact on virulence in mice.
作者信息
Chiavolini Damiana, Memmi Guido, Maggi Tiziana, Iannelli Francesco, Pozzi Gianni, Oggioni Marco R
机构信息
Dipartimento di Biologia Molecolare, Laboratorio di Microbiologia Molecolare e Biotecnologia, Università di Siena, Siena, Italy.
出版信息
BMC Microbiol. 2003 Jul 3;3:14. doi: 10.1186/1471-2180-3-14.
BACKGROUND
Streptococcus pneumoniae possesses large zinc metalloproteinases on its surface. To analyse the importance in virulence of three of these metalloproteinases, intranasal challenge of MF1 outbred mice was carried out using a range of infecting doses of wild type and knock-out pneumococcal mutant strains, in order to compare mice survival.
RESULTS
Observation of survival percentages over time and detection of LD50s of knock out mutants in the proteinase genes in comparison to the type 4 TIGR4 wild type strain revealed two major aspects: i) Iga and ZmpB, present in all strains of S. pneumoniae, strongly contribute to virulence in mice; (ii) ZmpC, only present in about 25% of pneumococcal strains, has a lower influence on virulence in mice.
CONCLUSIONS
These data suggest Iga, ZmpB and ZmpC as candidate surface proteins responsible for pneumococcal infection and potentially involved in distinct stages of pneumococcal disease.
背景
肺炎链球菌表面存在大型锌金属蛋白酶。为分析其中三种金属蛋白酶在毒力方面的重要性，使用一系列感染剂量的野生型和基因敲除肺炎球菌突变株对MF1远交系小鼠进行鼻内攻击，以比较小鼠的存活率。
结果
观察随时间变化的存活百分比，并检测蛋白酶基因敲除突变体与4型TIGR4野生型菌株相比的半数致死剂量（LD50），揭示了两个主要方面：i）存在于所有肺炎链球菌菌株中的Iga和ZmpB对小鼠毒力有强烈影响；ii）仅存在于约25%肺炎球菌菌株中的ZmpC对小鼠毒力的影响较小。
结论
这些数据表明Iga、ZmpB和ZmpC是负责肺炎球菌感染并可能参与肺炎球菌疾病不同阶段的候选表面蛋白。
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