Planchon P, Magnien V, Ronco G, Villa P, Brouty-Boye D
Institut d'Oncologie Cellulaire et Moléculaire Humaine, Bobigny, France.
Anticancer Res. 1992 Nov-Dec;12(6B):2315-20.
A new stable butyrate derivative monobut-3 was previously shown to inhibit proliferation and promote differentiation in human mammary established cell lines. The present study on monobut-3's effects on mammary epithelial cells cultured from human non-malignant and malignant breast tissues demonstrated pronounced morphological alterations suggestive of cellular differentiation. In addition, some degree of architectural differentiation was also evident in treated primary cultures. Monobut-3 did not affect the expression of vimentin and cytokeratin 18 when assessed in human breast cell lines expressing one or both types of intermediate filaments. However, it did induce expression of cytokeratin 19, characteristic of fully differentiated mammary cells, in one of the two cell lines devoid of this cytokeratin subtype. Furthermore, the network of intermediate filaments was often more largely extended in cells treated with monobut-3 than in untreated ones. These results indicate that monobut-3 can induce subtle changes in intermediate filaments which may contribute to its ability to promote differentiation in human mammary cells.
一种新的稳定丁酸衍生物单丁-3先前已被证明可抑制人乳腺成熟细胞系的增殖并促进其分化。目前关于单丁-3对从人非恶性和恶性乳腺组织培养的乳腺上皮细胞作用的研究表明，其有明显的形态学改变，提示细胞分化。此外，在处理过的原代培养物中也明显存在一定程度的结构分化。当在表达一种或两种类型中间丝的人乳腺细胞系中评估时，单丁-3不影响波形蛋白和细胞角蛋白18的表达。然而，在缺乏这种细胞角蛋白亚型的两个细胞系之一中，它确实诱导了完全分化的乳腺细胞所特有的细胞角蛋白19的表达。此外，与未处理的细胞相比，用单丁-3处理的细胞中的中间丝网络通常延伸得更大。这些结果表明，单丁-3可诱导中间丝发生细微变化，这可能有助于其促进人乳腺细胞分化的能力。
