Benshalom G
Department of Morphology, Corob Center for Health Sciences, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel.
Microsc Res Tech. 1992 Dec 1;23(4):324-33. doi: 10.1002/jemt.1070230407.
The combined light and electron microscopic analysis of Golgi-impregnated neural tissue is a potent tool for determining the connectivity of neural networks within the brain. In the experimental paradigms commonly applied in these studies, the Golgi-impregnated neurons are typically examined as the postsynaptic neuronal components. The structural characteristics and the pattern of distribution of their synaptic connections with other groups of identified neurons are analyzed. Due to the high power of resolution of the Golgi-electron microscopic technique, the ultrastructural analysis of Golgi-impregnated neurons can be expanded to elucidate activity-dependent structural alterations in their cytoarchitecture. These structural alterations can then be correlated under different physiological conditions with changes in the functional efficacy of the subcellular neuronal components.
对高尔基染色的神经组织进行光镜和电镜联合分析，是确定脑内神经网络连接性的有力工具。在这些研究中常用的实验范式里，通常将高尔基染色的神经元作为突触后神经元成分进行检查。分析它们与其他已识别神经元群体的突触连接的结构特征和分布模式。由于高尔基电镜技术的高分辨率，对高尔基染色神经元的超微结构分析可以扩展，以阐明其细胞结构中依赖活动的结构改变。然后可以将这些结构改变在不同生理条件下与亚细胞神经元成分功能效能的变化相关联。
