Bourne James A, Rosa Marcello G P
Department of Physiology, Monash University, Clayton, VIC 3800, Australia.
Brain Res Brain Res Protoc. 2003 Jul;11(3):168-77. doi: 10.1016/s1385-299x(03)00044-8.
The marmoset is becoming an important model for studies of primate vision, due to factors such as its small body size, lissencephalic brain, short gestational period and rapid postnatal development. For many studies of visual physiology (including single-cell recordings), it is a requirement that the animal is maintained under anaesthesia and neuromuscular block in order to ensure ocular stability. However, maintaining such a small animal (290-400 g) in good physiological condition for long periods requires expert attention. This becomes particularly important in the case of recordings from visual association cortex, where neuronal responses are known to be highly sensitive to factors such as the type and dose of anaesthetic, and the animal's physiological balance. The present protocol summarises our laboratory's experience over the last decade in developing a preparation for the study of marmoset visual cortex. It allows excellent recording from extrastriate areas for periods of at least 48 h, including the continuous study of isolated single cells for several hours.
由于体型小、大脑平滑、妊娠期短和出生后发育迅速等因素，狨猴正成为灵长类视觉研究的重要模型。对于许多视觉生理学研究（包括单细胞记录），要求动物在麻醉和神经肌肉阻滞状态下维持，以确保眼球稳定。然而，长时间将如此小的动物（290 - 400克）维持在良好的生理状态需要专业的照料。在从视觉联合皮层进行记录时，这一点尤为重要，因为已知神经元反应对麻醉剂的类型和剂量以及动物的生理平衡等因素高度敏感。本方案总结了我们实验室在过去十年中开发狨猴视觉皮层研究准备方法的经验。它允许在至少48小时内从纹外区域进行出色的记录，包括对单个分离细胞进行数小时的连续研究。
