Grass Stefan, Jacoby Arie S, Iismaa Tiina P, Crawley Jacqueline N, Xu Xiao-Jun, Wiesenfeld-Hallin Zsuzsanna
Department of Laboratory Medicine, Division of Clinical Neurophysiology, Huddinge University Hospital, Karolinska Institutet, S-141 86 Stockholm, Sweden.
Neurosci Lett. 2003 Jul 24;345(3):153-6. doi: 10.1016/s0304-3940(03)00516-0.
This study was conducted to examine the excitability of the nociceptive flexor reflex and its sensitization by repetitive stimulation of C-fibers in anesthetized mice that lack the galanin-R1 receptor. Repetitive stimulation of C-fibers induced a gradual increase in reflex magnitude during the stimulation (wind-up), and a subsequent increase in spinal reflex excitability (central sensitization). This occurred in GAL-R1 -/-, GAL-R1 +/-, and +/+ wild-type controls, with no significant differences observed between genotypes. Intrathecal administration of galanin markedly blocked the sensitization following the repetitive stimulation in all three groups. No differences between wild-type or galanin-R1 receptor knockout mice were seen. These results confirm previous studies in rats, showing that intrathecal galanin reduces the central sensitization following wind-up. The present data indicate that this effect is probably mediated by receptors other than GAL-R1.
本研究旨在检测缺乏甘丙肽-R1受体的麻醉小鼠中伤害性屈肌反射的兴奋性及其通过重复刺激C纤维引起的敏化作用。重复刺激C纤维在刺激过程中诱导反射幅度逐渐增加（卷绕），随后脊髓反射兴奋性增加（中枢敏化）。这在GAL-R1 -/-、GAL-R1 +/-和+/+野生型对照小鼠中均发生，各基因型之间未观察到显著差异。鞘内注射甘丙肽在所有三组中均显著阻断重复刺激后的敏化作用。野生型或甘丙肽-R1受体敲除小鼠之间未见差异。这些结果证实了先前在大鼠中的研究，表明鞘内注射甘丙肽可减轻卷绕后的中枢敏化。目前的数据表明，这种作用可能由GAL-R1以外的受体介导。
