van Leeuwen Willem B, Jay Corinne, Snijders Susan, Durin Nathalia, Lacroix Bruno, Verbrugh Henry A, Enright Mark C, Troesch Alain, van Belkum Alex
Department of Medical Microbiology & Infectious Diseases, Erasmus MC, 3015 GD Rotterdam, The Netherlands.
J Clin Microbiol. 2003 Jul;41(7):3323-6. doi: 10.1128/JCM.41.7.3323-3326.2003.
A newly developed oligonucleotide array suited for multilocus sequence typing (MLST) of Staphylococcus aureus strains was analyzed with two strain collections in a two-center study. MLST allele identification for the first strain collection fully agreed with conventional strain typing. Analysis of strains from the second collection revealed that chip-defined MLST was concordant with conventional MLST. Array-mediated MLST data were reproducible, exchangeable, and epidemiologically concordant.
在一项双中心研究中,使用两个菌株库对新开发的适用于金黄色葡萄球菌菌株多位点序列分型(MLST)的寡核苷酸阵列进行了分析。第一个菌株库的MLST等位基因鉴定结果与传统菌株分型完全一致。对第二个菌株库的菌株分析表明,芯片定义的MLST与传统MLST一致。基于阵列的MLST数据具有可重复性、可交换性且在流行病学上具有一致性。