Labeling during cleavage of nucleic acids for their detection on DNA chips.
作者信息
Laayoun A, Sothier I, Menou L, Bernal-Méndez E, Kotera M, Bourget C, Trévisiol E, Lhomme J, Troesch A
机构信息
BioMérieux, Advanced Technology, Chemin de l'Orme, Marcy l'Etoile, France.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):649-52. doi: 10.1081/NCN-120022603.
PMID:14565245
Abstract
A new and efficient strategy for labeling of nucleic acids prior to their hybridization on high density DNA chip has been developed. Our approach which combines the fragmentation and the labeling is based on the reactivity of the terminal phosphates of cleaved DNA and RNA fragments with a reporter molecule bearing aryldiazomethane group.
摘要
Zotero 插件