Shioda S, Shimoda Y, Nakai Y
Department of Anatomy, Showa University School of Medicine, Tokyo, Japan.
Neurosci Lett. 1992 Dec 14;148(1-2):155-8. doi: 10.1016/0304-3940(92)90828-u.
Direct projections from the A1/C1 catecholaminergic cell group in the caudal ventrolateral medulla oblongata to neurons containing vasopressin (VP) in the supraoptic nucleus (SON) were studied electron microscopically by a double-labeling technique which combines anterograde tracing after injection of wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP) into the A1/C1 cell group with VP immunocytochemistry. WGA-HRP-labeled axon terminals originating from neurons in the A1/C1 cell group were found to make synaptic contacts with VP-immunoreactive cell bodies and dendrites in the SON, most often forming axo-dendritic synapses. This indicates that VP-containing neurosecretory neurons in the SON receive monosynaptic catecholaminergic input from the A1/C1 cell group.
通过一种双重标记技术对延髓尾端腹外侧A1/C1儿茶酚胺能细胞群向视上核（SON）中含血管加压素（VP）的神经元的直接投射进行了电子显微镜研究，该技术将向A1/C1细胞群注射小麦胚芽凝集素结合辣根过氧化物酶（WGA-HRP）后的顺行追踪与VP免疫细胞化学相结合。发现源自A1/C1细胞群神经元的WGA-HRP标记轴突终末与SON中VP免疫反应性细胞体和树突形成突触联系，最常见的是形成轴突-树突突触。这表明SON中含VP的神经分泌神经元接受来自A1/C1细胞群的单突触儿茶酚胺能输入。
