[STI571对急性非淋巴细胞白血病患者骨髓细胞中c-kit表达的体外作用]

[In vitro effect of STI571 on expression of c-kit in bone marrow cells from patients with acute non-lymphocytic leukemia].

作者信息

Tan Tai Lin-Fang, Yi Zheng-Shan, Feng Ru

机构信息

Department of Hematology, Nanfang Hospital, The First Military Medical University, Guangzhou, 510515 China.

出版信息

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2003 Jun;11(3):256-8.

PMID:12844407

Abstract

This study was designed to explore the influence of STI571, a tyrosine kinase inhibitor, on the expression of c-kit in the bone marrow cells from patients with acute non-lymphocytic leukemia (ANLL). The cells were exposed to various concentration of STI571 for 72 hours, the expression of c-kit mRNA and CD117 was assayed by RT-PCR and flow cytometry, respectively. The results showed that STI571 treatment induced concentration-dependent decrease of c-kit and CD117 expression, which was significant lower than that in group before treatment and untreated control groups (P < 0.05) and 0.1 micro mol/L STI571 group was significantly higher than that in 10 micro mol/L group (P < 0.05). It is concluded that STI571 has an obvious effect on the restraint of c-kit in ANLL cells.

摘要

本研究旨在探讨酪氨酸激酶抑制剂STI571对急性非淋巴细胞白血病（ANLL）患者骨髓细胞中c-kit表达的影响。将细胞暴露于不同浓度的STI571中72小时，分别通过逆转录聚合酶链反应（RT-PCR）和流式细胞术检测c-kit mRNA和CD117的表达。结果显示，STI571处理可诱导c-kit和CD117表达呈浓度依赖性降低，显著低于治疗前组和未处理的对照组（P<0.05），且0.1微摩尔/升STI571组显著高于10微摩尔/升组（P<0.05）。结论是，STI571对ANLL细胞中c-kit的抑制作用明显。