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Overexpression and purification of Saccharomyces cerevisiae DNA topoisomerase II from yeast.

作者信息

Lindsley J E

机构信息

Department of Biochemistry, University of Utah, School of Medicine, Salt Lake City, USA.

出版信息

Methods Mol Biol. 1999;94:187-97. doi: 10.1385/1-59259-259-7:187.

DOI:10.1385/1-59259-259-7:187
PMID:12844875
Abstract
摘要

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Overexpression and purification of Saccharomyces cerevisiae DNA topoisomerase II from yeast.从酵母中过表达和纯化酿酒酵母DNA拓扑异构酶II
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Varying levels of positive and negative supercoiling differently affect the efficiency with which topoisomerase II catenates and decatenates DNA.不同程度的正超螺旋和负超螺旋对拓扑异构酶II使DNA连环化和解连环化的效率有着不同的影响。
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