Gliemroth Jan, Feyerabend Thomas, Gerlach Christiane, Arnold Hans, Terzis A Jorge A
Department of Neurosurgery, Medical University of Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Germany.
Neurosurg Rev. 2003 Jul;26(3):198-205. doi: 10.1007/s10143-003-0253-1. Epub 2003 Feb 12.
Radiotherapy is a well established treatment for malignant gliomas. This study describes the migration, proliferation, and invasion behaviour of two human glioma cell lines (GaMg and U-87 Mg) grown as multicellular tumour spheroids after radiotherapy. Migration and proliferation studies were performed using conventional and accelerated fractionation up to 60 Gy and 59.4 Gy, respectively. A dose-dependent growth and migratory response to irradiation independent of the type of fractionation was observed. A coculture system in which tumour spheroids were confronted with foetal rat brain aggregates was used for invasion studies. Marked invasion of the glioma spheroids into the brain aggregates occurred with or without radiotherapy. For the GaMg cells, flow cytometric DNA histograms after treatment with 10 Gy and 40 Gy showed an accumulation of cells in the G2/M phase of the cell cycle. Radiotherapy inhibits tumour cell growth and migration, but the invasiveness of the remaining tumour cells seems to be unaffected.
放射疗法是治疗恶性胶质瘤的一种成熟方法。本研究描述了两种人类胶质瘤细胞系（GaMg和U - 87 Mg）在放射治疗后作为多细胞肿瘤球体生长时的迁移、增殖和侵袭行为。迁移和增殖研究分别使用常规分割和加速分割，剂量分别高达60 Gy和59.4 Gy。观察到对辐射的剂量依赖性生长和迁移反应，与分割类型无关。使用肿瘤球体与胎鼠脑聚集体接触的共培养系统进行侵袭研究。无论是否进行放射治疗，胶质瘤球体均显著侵袭脑聚集体。对于GaMg细胞，用10 Gy和40 Gy处理后的流式细胞术DNA直方图显示细胞在细胞周期的G2/M期积累。放射治疗可抑制肿瘤细胞的生长和迁移，但剩余肿瘤细胞的侵袭性似乎未受影响。
